Repairing the spinal cord with activated fat

Activated Fat was developed by the University of Milan after several years of research funded by Neurogel Running. The activated fat comes from the fatty tissue of the patient. This adipose tissue contains stem cells, in particular mesenchymal stem cells. The innovative aspect of the therapy was to use adipose tissue as a three-dimensional matrix for cell growth.
   La graisse activée provient du tissu adipeux du patient.
   Ce tissu adipeux contient des cellules souches notamment des cellules souches mésenchymateuses.
   L’aspect novateur de la thérapie a consisté à utiliser le tissu adipeux comme matrice tridimensionnelle de croissance cellulaire.
   A specific mechanical activation of adipose tissue confers on the adipose tissue and the stem cells it contains clearly amplified natural characteristics:
      longer life in an ischemic environment (poor circulation and lack of oxygen)
      highly amplified anti-inflammatory factors
      the key expression of genes involving the regeneration of cell tissue
      immunosuppressive factors that block growth inhibitors

Mesenchymal stem cells from adipose tissue

It is mainly in bones and fatty tissue that stem cells are found which are able to recreate other cells in the human body. These cells are called the mesenchymal stem cells discovered in the early 1990s. Mesenchymal stem cells from adipose tissue have been the subject of significant research for several years. The fatty tissue is easy to extract and without risk to the patient. It is also relatively easy to extract mesenchymal stem cells from fatty tissue. They are also found to be able to generate in culture different cells of muscle, bones, tendons and neuronal cells Unfortunately, mesenchymal stem cells are indeed capable of differentiating into muscle, cartilage, bone and nerve cells IN VITRO, but attempts in VIVO do not give the expected results. These cells do not survive long enough and do not have the time or capacity to properly generate new cell tissue. Different cultivation methods are implemented and tested around the world to overcome this problem.es os et le tissu adipeux que l’on trouve des cellules souches qui sont capables de recréer d’autres cellules du corps humain.
 On appelle ces cellules, les cellules souches mésenchymateuses découvertes au début des années 90.
Les cellules souches mésenchymateuses issues du tissu adipeux font l’objet, depuis quelques années déjà, d’une importante recherche. Le tissu adipeux est facile à extraire et sans risque pour le patientIl est relativement facile aussi d’extraire les cellules souches mésenchymateuses du tissu adipeux. Il s’avère aussi que celles-ci sont capables de générer en culture différentes cellules du muscle, des os, des tendons  et des cellules neuronales
Malheureusement les cellules souches mésenchymateuses sont certes capables de se différencier en muscle, cartilage, os et cellules nerveuses IN VITRO, mais les tentatives in VIVO ne donnent pas les résultats escomptés. Ces cellules ne survivent pas assez longtemps et n’ont pas le temps ni la capacité de générer correctement un nouveau tissu cellulaire.
Différentes méthodes de culture sont mises en œuvre et testées dans le monde pour pallier à ce problème.

The concept of mechano-transduction with activated fat

In studies conducted by the University of Milan, the basic principle was to leave the mesenchymal stem cells in their natural environment: adipose tissue. By applying various orbital and helical forces to adipose tissue under a certain pressure and defined times, a phenomenon of tissue stress is created which will generate a cascade of events within the adipose tissue. The mesenchymal stem cells will then develop regenerative and regulatory capacities of the tissue while also stopping inflammation. They will act as a control tower and again express the key factors of tissue regeneration. This action on cells by mechanical forces is called mechanotransduction. The cells then overexpress genes and cytokines that are hardly found in adipose tissue. This mechanical action changes tissue expression without genetic modification. This is called epigenetics. le principe de base a consisté à laisser les cellules souches mésenchymateuses dans leur milieu naturel: le tissu adipeux.
En appliquant au tissu adipeux diverses forces orbitales, hélicoïdales, sous une certaine pression et des temps définis, on crée un phénomène de stress tissulaire qui va engendrer une cascade d’évènement au sein du tissu adipeux.
Les cellules souches mésenchymateuses vont alors développer des capacités de régénération et de régulation du tissu en stoppant également l’inflammation.
Elles vont agir en tour de contrôle et exprimer à nouveau les facteurs clés de la régénération tissulaire.
Cette action sur les cellules par des forces mécaniques est appelé mécanotransduction. Les cellules surexpriment alors des gênes et des cytokines qu’on ne retrouve quasiment pas dans le tissu adipeux.
Cette action mécanique change l’expression tissulaire sans modification génétique. On appelle cela l’épigénétique.

Some scientific data on the properties of fat with or without activation

To learn more about the subject, you can read Michèle Zander's translation of the International article Journal of molecular Sciences, including the table on page 4. This is a simplified document on the activation of fat tissue.

 

So with this mechanotransduction treatment induced via these cells, here is what happens:
High levels of IL-15 then directly inhibit adipogenesis, but for fat in this case high levels of IL-15 make the tissue highly IN VIVO IMMUNOSUPPRESSOR.
High levels of TSG-6 also decrease adipogenesis but also increase in fat with powerful effects IN VIVO ANTI-INFLAMMATORY.
Significant levels of OCT4 and SOX2 are essential transcription factors for CELLULAR RENEWAL.
With high levels of nano-g hADSCs are able to survive, migrate to damaged sites, and become NEURAL STEM CELLS AFTER TRANSFORMATION AND THIS IN VIVO.

So here we are with a so-called activated fat in which we actually find:

A new kind of neural stem cells which are resistant to growth inhibitors by powerful immunosuppressive factors.
New neural stem cells that induce neuroprotection by powerful anti-inflammatory factors.
New neural stem cells capable of multiplying in vivo and differentiating into neurons and / or oligodendrocytes.

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